Influence of FSH Treatment on Expression of CDC25A, TSSK3 and P53 in Vitro Cultured Sertoli Cells of Calf

Abstract CDC25A, TSSK3 and P53 expressions in vitro in cultured sertoli cells after FSH treatment were studied in order to provide some data for further researches of spermatogenesis. Different concentrations of FSH (0, 0.01, 0.02, 0.04, and 0.08 IU. mL -1 ) were used to treat sertoli cells cultured in vitro. The expression of CDC25A, TSSK3 and P53 was determined by real-time-PCR at 6 h, 12 h and 24 h after FSH treatment of sertoli cells. The results showed that FSH had no significant effect on expression of CDC25A ( p >0.05), could significantly improve the expression of TSSK3 and P53 ( p