Research on protective function of aspirin-triggered lipoxins on acute kidney injury in mice

Objective To investigate the renoprotective effect of aspirin-triggered lipoxins (ATL) on kidney of mice with acute kidney injury(AKI). Methods Eighty-eight male specific pathogen-free(SPF) C57BL/6J mice were randomly divided into lipopolysaccharide (LPS) groups(including 2 h group, 4 h group, 8 h group, 12 h group, 24 h group), ATL+ LPS(including 2 h group, 4 h group, 8 h group, 12 h group, 24 h group)and normal control group according to random numble table, and each group had 8 mice.The mice in LPS groups were given LPS intraperitoneal injection to establish AKI animal models, while the mice in ATL+ LPS groups were given ATL intraperitoneal injection 30 minutes before LPS intraperitoneal injection.The enzyme linked immunosorbent assay was used to test the serum creatinine(Scr), serum urea nitrogen(BUN), tumor necrosis factor-α(TNF-α), interleukin-1β(IL-1β) and urine neutrophil gelatinase-associated lipocalin(NGAL), kidney injury molecule-1(KIM-1), cysteine-rich protein-61(Cyr61) and netrin-1 levels of mice. Results The kidney tissue injury scores of mice of ATL+ LPS group[4 h: (22.32±1.04) scores, 8 h: (31.11±1.86) scores, 12 h: (18.22±0.92) scores, 24 h: (20.87±3.18) scores] were lower than those of LPS group at the corresponding time points [4 h: (35.47±2.27) scores, 8 h: (52.28±2.82) scores, 12 h: (54.99±4.56) scores, 24 h: (53.41±4.76) scores], and the differences were statistically significant (all P<0.01). The values of Scr, BUN, TNF-α and IL-1β in ATL+ LPS group[Scr 8 h: (143.07±5.02) μmol/L, BUN 12 h: (33.07±3.52) mmol/L, TNF-α 4 h: (196.33±14.181) ng/L and 8 h: (221.77±10.11) ng/L, IL-1β 4 h: (50.25±2.67 ng/L)] were lower than those in LPS group at the corresponding time points [Scr 8 h: (227.43±11.17) μmol/L, BUN 12 h: (59.68±3.84) mmol/L, TNF-α 4 h: (267.87±26.48) ng/L and 8 h: (334.78±21.08) ng/L, IL-1β 4 h: (89.45±5.87) ng/L], and the differences were statistically significant (all P<0.01). The urine NGAL [4 h: (56.76±4.01) μg/L, 8 h: (65.44±7.81) μg/L], KIM-1[8 h: (78.19±9.48) μg/L] and netrin-1[8 h: (40.12±2.01) ng/L, 12 h: (36.87±2.87) ng/L] of mice in ATL+ LPS group were lower than those in LPS group at the corresponding time points [NGAL 4 h: (168.77±10.77) μg/L, 8 h: (155.33±8.26) μg/L; KIM-1 8 h: (124.73±13.47) μg/L; netrin-1 8 h: (89.17±2.74) ng/L, 12 h: (81.11±3.88) ng/L], and the differences were statistically significant(all P<0.01). Conclusions ATL can treat LPS-induced AKI and play a renoprotective role in the kidney. Key words: Aspirin-triggered lipoxins; Lipopolysaccharide; Kidney injury, acute; Kidney