Crystal Structure of 5ʹ-Oxospiro-(fluorene-9,4ʹ-imidazolidine)-2ʹ-thione and Biological Activity of Its Derivatives

The presented study is devoted to the structural and biological properties of four derivatives of fluorenylspirohydantoin based on experimental data. The crystal structure of one of those, (9ʹ-fluorene)-spiro-5-(2-thiohydantoin), is described. The compound crystallizes in monoclinic crystal system and P21/c space group. We have pursued isolation of single crystals and biological activity tests of (9ʹ-fluorene)-spiro-5-hydantoin, (9ʹ-fluorene)-spiro-5-(2,4-dithiohydantoin), (9ʹ-fluorene)-spiro-5-(2-thiohydantoin), and (4ʹ,5ʹ-diaza-9ʹ-fluorene)-spiro-5-hydantoin. The compounds have been tested in both adherent and suspension tumor cell lines. The cell proliferation has been assessed by the WST-assay (Roche Applied Science). The antimicrobial activity against both Gram-positive, Gram-negative bacteria, and the yeast Candida albicans has been studied.