cells during acute toxoplasmosis. characteristics of human gamma and delta T Phenotypic profile and functional

were used, a second incubation with phycoerythrin-conju-gated rat anti-mouse serum was performed. Fluorescencewas measured on a FACScan flow cytometer. Controlsincludedfluorescein isothiocyanate-orphycoerythrin-conju-gated mouseimmunoglobulins. Cells (10,000) wereacquiredin the list modeofthe Consort 30 computer.Mononuclearcells wereseparated after Ficoll-Paque cen-trifugation. Cells were incubated for 30 min at 4°C withappropriate amountsofanti-CD4, anti-CD8, anti-CD16, andanti-CD19 monoclonal antibodies; after being washed, thecells