Paraburkholderia tropica as a plant-growth–promoting bacterium in barley: characterization of tissues colonization by culture-dependent and -independent techniques for use as an agronomic bioinput

Aims Characterization of barley-tissue–colonization efficiency (Hordeum vulgare L.) by Paraburkholderia tropica MTo-293 after seed inoculation was studied in two plant-growth systems: (1) in flasks with semisolid agar-containing sterile medium, as a suitable environment to study plant-bacteria interaction and also optimize molecular-biology techniques, and (2) in a pot-substrate system as an approach to understand their potential behavior as bioinput.