Embryo-maternal communication in dogs: Immune system related factors

Abstract In the bitch, establishment of pregnancy is believed to be mainly initiated by the free-floating embryo in the uterus that is under progesterone influence. As in other species, the active participation of the embryo is no longer questioned. Secretory products are transported to the embryo-maternal interface and contribute to extra-cellular matrix (ECM) degradation, a change in the intrauterine immune milieu towards a reduction of immune cells and a change in lymphocyte subsets, cell differentiation, angiogenesis, and the balance between proliferation and apoptosis. For cell-to-cell communication between embryo and maternal tissue, biomolecules inclusive microRNAs might be transported and exchanged via extracellular vesicles (EVs) as in other species. Maternal acceptance of the fetal allograft is vital for the establishment of pregnancy. Findings so far indicate that the embryo avoids attacks from the maternal system via passive and active mechanisms. One hypothesis is that expression or suppression of surface molecules help the canine embryo to hide from the maternal immune system on one side and to actively destroy cytotoxic immune cells on the other side; there are further clues that the canine embryo blocks activation of intrauterine leukocytes. Intracellular repair mechanisms via heat shock proteins (HSP) are candidates under investigation. The presence and function of immunomodulatory intrauterine cells like Treg cells and their interaction with the embryo have been intensely studied in other species but remains to be investigated in the canine preimplantation uterus.