Gene diagnostics of gender verification test in competitive sports

: The gender verification test (GV) was carried out by gene diagnostics at the 12th Asian Games in Hiroshima, 1994. Human genomic DNA samples were extracted from the hair root of female competitors. Polymerase chain reaction (PCR) was used to prove the absence of the sex determining region of Y-chromosome (SRY) in order to confirm the femininity, and detection of pseudo autosomal boundary region of Y-chromosome (PABY) in order to confirm that the DNA is extracted from the hair root and the femininity can again be confirmed. A total of 1,300 female competitors were screened. We conclude that this testing is useful for the GV, because hair sampling is easy, the method is simple and there is little contamination.