A kind of nucleic acid detection method based on magnetic bead Yu ultrasonic Separation luminous marker

The present invention relates to a kind of nucleic acid detection method based on magnetic bead Yu ultrasonic Separation luminous marker: the method is at magnetic bead surfaces rhetorical function arm molecule；It is designed for detecting the functionalization probe of target nucleic acid fragment, by functionalization probe modification in the magnetic bead surfaces being combined with functionalization arm molecule；By hybridization, target nucleic acid fragment and luminous marker thereof are captured to magnetic bead surfaces；Magneto separate, cleans, has been obtained the magnetic bead of luminous marker；By physical methods such as ultrasonic wave process, luminous marker is separated from magnetic bead surfaces, the luminous marker solution without magnetic bead is carried out luminous detection, obtains target nucleic acid information.The present invention can not only overcome the luminous signal loss that the capture-effect of luminous signal is caused by magnetic bead, and use the physics modes such as ultrasonic wave process, the reagent that chemistry or biological method can be avoided to cause inactivates or other interference, being capable of detecting when minimum 50amol nucleic acid fragment in 20 μ L volumes, detection sensitivity is high.