Establishment of UPLC Fingerprints of Hovenia dulcis Thunb and Hovenia acerla Thunb and Analysis of Its Characteristic Components

OBJECTIVE To establish the UPLC fingerprints of Hovenia dulcis Thunb and Hovenia acerla Thunb,and to identify the characteristic chemical components in the fingerprint by UPLC-Q-TOF-MS. METHODS The chromatographic fingerprint was obtained with ACQUITY UPLC HSS T3 column( 100 mm × 2. 1 mm,1. 8 μm) and gradient eluted with acetonitrile and 0. 2% formic acid; The flow rate was 0.15 m L/min,and the column temperature was maintained at 25 ℃. The detection wavelength was set at 340 nm. The mass spectrometer was operated in negative mode. RESULTS The UPLC fingerprints of Hovenia dulcis Thunb and Hovenia acerla Thunb were established to identify 12 compositions for 11 common peaks. The similarity of fingerprints of Hovenia dulcis Thunb was more than 0. 89 and the similarity of fingerprints of Hovenia acerla Thunb was more than 0. 81 except 1 batch of sample.CONCLUSION The method is accurate and reproducible,and it can provide a reference for the quality control of medicines.