Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards

Abstract We evaluated the limits of detection (LoD) for an 11-plex PCR-Luminex assay performed on Whatman™ FTA Elute cards smeared with stool containing pathogens associated with travelers' diarrhea. LoDs ranged from 10 2 to 10 5  CFU, PFU, or cysts/g for most pathogens except Cryptosporidium. Campylobacter and norovirus LoDs increased with prolonged storage of cards.