The complete chloroplast genome of Chinese medicine (Psoralea corylifolia): Molecular structures, barcoding and phylogenetic analysis

Abstract Psoralea corylifolia is one kind of traditional Chinese medicine used in China widely. In this study, we sequence the complete chloroplast genome of P. corylifolia, which is 153,114 bp in size and includes a pair of inverted repeats regions of 25,557 bp interspersed by a small single copy of 17,885 bp and a large single copy of 84,115 bp region. Approximately 98 simple sequence repeats, 14 forward, 2 reverse, 2 complement, 32 palindromic and 49 tandem repeats are identified in the P. corylifolia chloroplast genome. The chloroplast genomes of P. corylifolia and three Glycine species are conserved in gene order and content, but show high diversity within intergenic spacers. P. corylifolia with three Glycine species in Papilionoideae fall into the same clade based on 75 conserved coding-protein genes phylogenomic analysis. Moreover, four chloroplast DNA regions (ycf1, matK, accD, ndhF) can serve as the barcodes. In general, our findings will dedicate to better comprehension of the genome aspect as well as evolutionary status of P. corylifolia.