Cloning and Expression Analysis of Glycogen Synthase Kinase gene ThbGSK and High-affinity K~+ Transporter gene ThbHKT1 of Thinopyrum bessarabicum

Thinopyrum bessarabicum Lve(2n = 2x = 14,JJ) represents an important genetic resource for wheat improvement.In this study,two full-length orthologous genes of glycogen synthase kinase(GSK) designated ThbGSK and high-affinity K+ transporters(HKT) designated ThbHKT1 were cloned from Th.bessarabicum leaf cDNA.In comparison with that of rice,Brachypondium distachyon and wheat,the identity of amino acid of the gene varied from 93.92%~92.02%,indicating high conservation of this gene in these species.Cluster analysis showed a closer relationship between ThbGSK and TaGSK1.In comparison with that of rice,wheat,Brachypondium distachyon and barley,the identity of amino acid of the gene ThbHKT1 varied from 66.17%~92.87%,indicating variation of this gene in these species.Cluster analysis showed a closer relationship between ThbGSK and HvGSK1.Stressed with 250 mmol·L-1 NaCl for 0~24 h,GSK showed constitutive expression patterns both in Th.bessarabicum and Chinese Spring while HKT1 showed up-regulated after semi-quantitative and quantitative analysis.