CHOLESTANE-TYPE STEROIDS FROM THE OCTOCORAL Verrucella corona

Using various chromatographic separations, three cholestane-type steroids were isolated from the methanol extract of the octocoral Verrucella corona. Their structures were elucidated to be (22 E )-cholesta-5,22-dien-3 β -ol-7-one ( 1 ), trans - liagosterol ( 2 ), and guggulsterol-II ( 3 ), by detailed analysis of the 1D and 2D-NMR data as well as comparison with those reported. Among them, compound 2 showed significant cytotoxicity against eight human cancer cell lines as HepG2, HL-60, KB, LNCaP, LU-1, MCF7, SK-Mel2, and SW480.