Protective effects of ginsenosides 20(R)-Rg3 on H2O2-induced myocardial cell injury by activating Keap-1/Nrf2/HO-1 signaling pathway.

2021 
Ginsenoside 20(S)-Rg3 and 20(R)-Rg3 are famous rare ginsenosides from red ginseng, and their configurations in C-20 are different. This study aimed to investigate the protective mechanism of ginsenoside 20(S)-Rg3, 20(R)-Rg3 on H 2 O 2 -induced H9C2 cells, and compare their activity. The results showed that the ginsenoside 20(S)-Rg3, 20(R)-Rg3 could increase the cell activity and the levels of GSH-Px, SOD and CAT, and decrease activities of LDH, MDA and ROS. Further studies showed that ginsenoside 20(S)-Rg3, 20(R)-Rg3 could prevent oxidative stress injury of H9C2 cells by H 2 O 2 through the Keap-1/Nrf2/HO-1 pathway. But the ML385 counteracts these effects. Interestingly, among these results, ginsenoside 20(R)-Rg3 was superior to 20(S)-Rg3, indicating that ginsenoside 20(R)-Rg3 have a stronger effect of anti-oxidative stress. This study reflected that ginsenoside 20(R)-Rg3 could be used as a potential Nrf2 activator and a safe effective Chinese herbal monomer in the treatment of CVD.
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