The triple-wavelength overlapping resonance Rayleigh scattering method and the fluorescence quenching method for the determination of chitooligosaccharides using trisodium-8-hydroxypyrene-1,3,6-trisulfonate as a probe

Abstract In this assay, the triple-wavelength overlapping resonance Rayleigh scattering (TWO-RRS) method and the fluorescence quenching method for the quantitative detection of chitooligosaccharides (COS) were developed. In the weakly Britton-Robinson buffer solution, COS interacted with Trisodium-8-hydroxypyrene-1,3,6-trisulfonate (HPTS) to form an ion-association complex of HPTS-COS, which increased the RRS intensities at 321 nm, 430 nm and 511 nm and decreased the fluorescence intensities of the system at 512 nm. And the changes in the intensities of both methods were related to the changes in the concentration of COS. Moreover, for the TWO-RRS method, OP-10 made the RRS intensities increased stronger, finally, the three peaks' total was linear to the concentration of COS in the range of 1.00–8.00 μg/mL and the limit of detection (LOD) was 0.247 μg/mL, and for the fluorescence quenching method, the linear range was 0.50–3.50 μg/mL with the LOD of 0.108 μg/mL. Based on these, two new and fast spectral methods with high sensitivity and simplicity for the determination of trace COS had been established. The generation mechanism of the TWO-RRS and the fluorescence quenching was studied. At the same time, the two methods were applied to the determination of COS in health products with satisfactory results.