Dual-model analysis for improving the discrimination performance of human and nonhuman blood based on Raman spectroscopy

The discrimination accuracy for human and nonhuman blood is important for customs inspection and forensic applications. Recently, Raman spectroscopy has shown effectiveness in analyzing blood droplets and stains with an excitation wavelength of 785 nm. However, the discrimination of liquid whole blood in a vacuum blood tube using Raman spectroscopy, which is a form of noncontact and nondestructive detection, has not been achieved. An excitation wavelength of 532 nm was chosen to avoid the fluorescent background of the blood tube, at the cost of reduced spectroscopic information and discrimination accuracy. To improve the accuracy and true positive rate (TPR) for human blood, a dual-model analysis method is proposed. First, model 1 was used to discriminate human-unlike nonhuman blood. Model 2 was then used to discriminate human-like nonhuman blood from the “human blood” obtained by model 1. A total of 332 Raman spectra from 10 species were used to build and validate the model. A blind test and external validation demonstrated the effectiveness of the model. Compared with the results obtained by the single partial least-squares model, the discrimination performance was improved. The total accuracy and TPR, which are highly important for practical applications, increased to 99.1% and 97.4% from 87.2% and 90.6%, respectively.