Accelerated solvent extraction - gel chromatography purification -lc / ms / ms bee pollen binding assay method nitroimidazole drugs

The present invention relates to a method for detecting pollen by the nitroimidazoles. Accelerated solvent extraction - gel chromatography purification -LC / MS / MS method bee pollen binding nitroimidazoles assay, the sample which was extracted with ethyl acetate solvent extraction Accelerated Solvent Extractor (ASE), for gel permeation chromatography purification (GPC), using LC-MS positive ion multiple reaction monitoring mode determination, matrix-matched standard curve isotope internal standard. Results: nitroimidazoles compound limit of detection was 1.5μg · kg-1, the limit of quantification was 5.0μg · kg-1. The recovery rate was 93.5% - 114.2%, relative standard deviation of 0.3 to 5.2%. Conclusion: This method has a high degree of automation and high sensitivity, accurate qualitative and quantitative results and so on.