Micropropagation of Kinnow mandarin using nodal segments as explants

In the present investigation nodal segments were used as explant for in vitro shoot regeneration in Kinnow. The Murashige and Skoog (1962) basal medium was fortified with BAP and NAA to examine the different shoot regeneration parameters. BAP (2.9 mg/L) and NAA (0.06 mg/L) when added together to the medium produced the maximum sprouting percent, number of shoots, shoot length, and number of leaves. The in vitro regenerated shoots were transferred in to half strength WPM medium enriched with IBA and NAA for initiation of root.