Allicin Reduces 5-fluorouracil-resistance in Gastric Cancer Cells through Modulating MDR1, DKK1, and WNT5A Expression.
2021
Background & Objective 5-fluorouracil (5-FU) is approved for the
treatment of gastric carcinoma (GC), but chemo-resistance limits the application
of it for GC. Thus, the combination of 5-FU with adjuvants such as allicin may
overcome multidrug resistance (MDR). Methods The anticancer effects of allicin, 5-FU, and allicin/5-FU
on the 5-FU resistant MKN-45 cells were evaluated by MTT assay and DAPi
staining. The expression of the P-glycoprotein (P-gp) and CD44 protein were
determined using immunocytochemistry. We also quantified mRNA expression levels
of WNT5A, Dickkopf-1 (DKK1), and MDR1 in the GC cells. Results Here, we found that the combination of allicin with 5-FU
significantly increased apoptosis compared to 5-FU alone
(P<0.05). We showed that WNT5A, MDR1, and
DKK1 mRNA expression levels were down-regulated in the allicin- and
allicin/5-FU-treated cells. Indeed, the combination of allicin and 5-FU
significantly decreased the expression of the P-gp and CD44 proteins
(P Conclusion Our findings indicate that the combination of allicin with 5-FU
could reverse multidrug resistance in the GC cells by reducing the expression of
WNT5A, DKK1, MDR1, P-gp, and CD44 levels.
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