Simultaneous measurement of optical rotation dispersion and absorption spectra for chiral substances.

We present a new method based on optical null methods for simultaneously measuring the optical rotatory dispersion (ORD) and absorption spectra of chiral substances. The optical rotation angle at a specific wavelength can be obtained from the optical nulls of the Malus curves with and without the sample. We use the optical nulls of the two curves as benchmark points and the readings to the right of the benchmark points by a certain angular offset to eliminate the influence of the analyzer on the light intensity and obtain the absorbance of the chiral substance at a specific wavelength. The 4096 pixels of the line scan CCD can measure multiple wavelengths simultaneously so that continuous ORD and absorption spectra can be obtained. The experimental results show that the standard deviation of the specific optical rotation is 0.11 deg mL g(-1) dm(-1) , the standard deviation of the maximum absorption wavelength is 0.45 nm, and that absorbance of the sample varies linearly with the concentration. This method is helpful for simplifying the experiment and has a profound influence on the analysis of the contents and molecular configurations of chiral substances in the future.