DEVELOPMENT OF A VERSATILESENSING MEMBRANE FORIMMUBILIZATION OF PROTEIN ANDA NEW IMMOBILIZATION METHOD

This paper describes a versatile sensing membrane for immobilizationof proteins (antibodies, antigens, enzymes and so on) and a new immobolizationmethod' The membrane was developed with polyacrylaminde gel and blotinstrep-tavidin system. Streptavidin was bond to the polyacryamide gel with 1-ethyl-3-(3-dimethylaminopropyl carbodimide (EDAC), which results in a streptavidin membrane, and a biotinalyted ligand was then easily linked to the gel via the streptavidin molecules.The streptavidin membrane (the thickness or 500urn) was used toimmobilize anti-human chorionic gonadotrophin antibody (Q hCG). The number orQbCG in the membrane was about 1011 per mm2 of the membrane surface. The membrane was used as a sensing membrane or optical immunosensor (Mach-Zehnder interferomter). It had a good response at the concentration or 10 afl/L hCG.