Identification of a lyssavirus isolate from a bat [Eptesicus serotinus]

The study describes identification of a lyssavirus isolate originating from a bat (Eptesicus serotinus) captured in Slovakia. The bat flew accidentally into a family house where it came into contact with a human. It was subsequently captured, euthanised and examined. Rabies antigen was detected by direct fluorescent antibody test (FAT), mouse inoculation test (MIT) and reverse transcriptase polymerase chain reaction (RT-PCR). By means of the direct sequencing of the amplification product of the nested reverse transcriptase polymerase chain reaction (nRT-PCR) gene of bat strain classified as a genotype 5 – EBL 1a was identified.