EFFECT OF TRANSPORT STRESS ON PERIPHERAL BLOOD LYMPHOCYTE SUBSETS AND Th CYTOKINES IN PIGS
2013
In order to investigate transport stress on porcine peripheral blood
lymphocyte subsets and Th cytokines, blood samples were collected from pigs
before and after transport. Creatine kinase (CK), alkaline phosphatase (ALP),
lactate dehydrogenase (LDH), glucose, and cortisol in the serum were
measured. The number of leukocytes and lymphocytes, percentages of lymphocyte
subsets, as well as Th cell cytokines level and their mRNA expression were
detected, respectively. After transport, the level of CK, glucose and
interleukin (IL)-4 increased significantly (p<0.01), LDH, Th memory cells,
natural killer and interferon (IFN)-γ increased significantly (p<0.05),
cortisol, number of leukocytes and lymphocytes decreased (p<0.01),
percentages of γ δ T cells, naive Th cells and cytotoxic T lymphocytes
decreased significantly (p<0.05). The mRNA expressions IL-2 and IFN-γ were
down regulated, p<0.01 and p<0.05, respectively. While IL-4, IL-6, and IL-10
were up regulated, but only IL-10 displayed a highly significant difference
(p<0.01). These data suggested that transport could cause immune suppression
in pigs, which influences cellular immunity more than humoral immunity, and
humoral immunity may play an important role in transport stress. Proper
measures should be taken to reduce susceptibility of infection after
transport.
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