Host range and complete genome sequence of Cucurbit chlorotic yellows virus, a new member of the genus Crinivirus.

ABSTRACT Cucurbit chlorotic yellows virus (CCYV) causes chlorotic yellows on cucumber (Cucumis sativus) and melon (Cucumis melo) and is transmitted by Bemisia tabaci biotype B and Q whiteflies. To characterize the host range of CCYV, 21 cucurbitaceous and 12 other plant species were inoculated using whitefly vectors. All tested Cucumis spp. except Cucumis anguria and Cucumis zeyheri were systemically infected with CCYV, although infection rates varied among species. Citrullus lanatus, Cucurbita pepo, and Luffa cylindrica were susceptible to CCYV; however, the infection rates were low and symptoms were unclear. In addition to the cucurbitaceous plants, Beta vulgaris, Chenopodium amaranticolor, Chenopodium quinoa, Spinacia oleracea, Lactuca sativa, Datura stramonium, and Nicotiana benthamiana were also systemically infected by CCYV. Complete RNA1 and RNA2 were reverse-transcribed, cloned, and sequenced. CCYV RNA1 was found to be 8,607 nucleotides (nt) long and contained four open reading frames (ORFs). The ...