Determination of ketamine in blood using gas chromatography/mass spectrometry(GC/MS) and gas chromatography/nitrogen phosphor detector (GC/NPD)

Objective To develop a qualitative and quantitative analysis method for the determination of ketamine in blood samples using solid-phase extraction combined with GC/MS and GC/NPD. Methods Using 4-PBA as the internal standard,and the samples were extracted by solid-phase extraction with Bond-Elut Certify cartridge. Analytes were eluted by dichloromethane :isopropanol :ammonium hydroxide (78:20:2,v/v/v). The optimum conditions for extraction,such as pH and eluent were studied in detail. Abuse drugs extracted from blood by solid phase extraction were detected and quantified by GC/MS and GC/NPD. Results The experimental results indicated that linear calibration curve were obtained within the range of 6.0～5000ng/mL,by GC/NPD and showed lower detection limit at 20.0ng/mL by GC/MS for ketamine in blood. The method recovery were 96.9%,and the standard deviation was less than 5%. Conclution The method may be applied for qualitative and quantitative analysis of ketamine in the drug abuse blood.