A sequential EMT-MET mechanism drives the differentiation of human embryonic stem cells towards hepatocytes

Reprogramming has been shown to involve EMT-MET; however, its role in cell differentiation is unclear. Here the authors show that during in vitro differentiation of hepatocytes, Activin A-induced formation of definitive endoderm requires EMT mediated by TGFβ signalling, followed by a MET process.