Effect of immunomodulators on serum nitric oxide concentration of endometritic repeat breeding crossbred cows

A study was carried out to evaluate the effect of immunomodulators on serum nitric oxide concentration of endometritic repeat breeding crossbred cows. The endometritic cows (n=60) were randomly divided into 6 groups (10 cows/group). In group A, E. coli LPS (100 μg in 30 ml PBS once), in group B, oyster glycogen (500 mg in 30 ml PBS once), in group C, whole colostrum (30 ml once), in group D, lyophilized colostrum (reconstituted in 30 ml PBS once), in group E, Cflox-Tz (60 ml for three consecutive days) and in group F (positive control), PBS (30 ml once) was infused through intrauterine route. Normal cyclic fertile animals (n=10) were served as negative control (Group G). Efficacy of these immunomodulators was assessed by drastic decline in the serum nitric oxide concentration at subsequent estrus. The results revealed that the cows with endometritis had significantly (p< 0.05) higher concentration of serum nitric oxide as compared to normal cyclic fertile cows. Following administration of E. coli LPS, oyster glycogen, whole colostrum and lyophilized colostrum, the serum nitric oxide concentration in endometritic cows decreased significantly (p< 0.05) at subsequent estrus. A significantly (p< 0.05) higher conception rate was observed in group A (70%), B (50%), C (50%), D (40%) and E (50%) as compared to group F cows (10%). It was concluded that E. coli LPS, oyster glycogen, whole colostrum and lyophilized colostrum stimulated the uterine defense mechanism and reduced oxidative stress in endometritic cows and might act as an alternative choice in treating endometritis against routine use of antibiotics.