Cytotoxicity of various extracts of Indigofera barberi Gamble

Indigofera barberi Gamble (Fabaceae) plant traditionally used to treat various skin diseases, renal disease and liver disease. Reported Phytochemicals are glycosides, steroids, tannins and Phenolic compounds and flavonoids. However, no studies have been investigated for cytotoxic effect. The present study carried out to investigate the cytotoxic effect of petroleum ether, chloroform and ethanolic extracts of this plant on the growth of the human liver hepatoma cell lines were examined by MTT assay. The IC50 value of petroleum ether, chloroform and ethanolic extracts of Indigofera barberi in HepG2 cell line found to be 150mg/mL, 100mg/mL and 75mg/mL respectively. Among the tested cell lines, ethanolic extract of was more selective cytotoxic against HepG2 cell line. The Compound IB-4, isolated by further subjected from ethanolic extracts and its IC50 value found to be 40mg/mL.