Eradication of Biofilm Formation by Crocus sativus Alcoholic Extract in Streptococcus mutans Clinical Isolates

Abstract Despite major advances in oral health in the past decades, but tooth decay is one of the most common preventable diseases in the worldwide. Now days, Streptococcus mutans is discussed as one of the most important challenges in tooth decay. In the other hand, medicinal herbs can be considered as an effective weapon against infectious diseases. The purpose of the current study was to investigate the biofilm formation in S. mutans clinical isolates and to evaluate the anti-biofilm properties of C. sativus against S. mutans clinical isolates. In this study, thirty dental plaque samples were collected from patients with referring to treatment centers of Hamadan University of Medical Sciences. Then, identification of samples was performed by standard methods. Biofilm formation was evaluated on S. mutans clinical isolates. Afterwards, C. sativus alcoholic extract was applied as an anti-biofilm formation in S. mutans clinical isolates. Our results demonstrated that a significant number of S. mutans samples were identified as strong, moderate and week biofilm producers. Then, C. sativus in 60 g/ml, 30 g/ml and 8 g/ml were able to eradicated strong, moderate and week biofilm formation in C. sativus, respectively. Therefore, the present study offered C. sativus as an anti-biofilm formation in S. mutans clinical isolates. In addition, more extensive studies and in vivo research are needed to confirm the results of this study.