Spectrometer for fluorescence–reflection biomedical research

A fluorescence–reflection multichannel fiber spectrometer has been developed that makes it possible to carry out a wide range of biomedical research, including estimating the concentration of glycation end-products in the skin and the oxidation–reduction state of various tissues. It has the feature of being able to simultaneously record diffuse reflection spectra and fluorescence emission. The fluorescence is excited by an LED that emits at 365 nm. The fluorescence spectra are recorded in the 400–750 nm region, while the reflection spectra are recorded in the 350–750 nm region. The convergence of the results of the measurements is 1.6%. A technique is proposed for correcting the autofluorescence index of the skin that makes it possible to sharply reduce its dependence on the degree of skin pigmentation. The possibilities of the device are illustrated by examples of biomedical research.