Gene expression profiling: Identification of genes with altered expression in Ayu17-449 knockout mice

Ayu17-449, a novel gene in mice, has been identi- fied as a tumor-suppressor gene in myeloid malignancy; its product catalyzes the conversion of 5-methylcytosine of DNA to 5-hydroxy- methylcytosine. However, in vivo, its functional target genes and biological function have remained unclear. Based on the assump- tion that alterations in the expression of the Ayu17-449 gene affect the expression of other related genes, we screened a microarray of altered gene expression in Ayu17-449 -/- and Ayu17-449 +/+ mice. We