Studies on quality standard of Wanxuanjing

Objective:To improve the method for quality control of Wanxuanjing. Methods:TLC was used to identify Cor-tex Kadsurae Radicis; the content of benzoic acid and salicylic acid in Wanxuanjing was determined by HPLC, the sample was separated by Shim-pack CLC-ODS C18 column(6.0 mm×150 mm,5 μm) using a mixture of MeOH-KH2PO4(50∶50) as the mobile phase, which was adjusted to pH 3.1 by phosphoric acid, the detective wavelength was set at 230 nm and the flow rate was 0.8 ml/min. Results:Cortex Kadsurae Radicis could be identified by TLC; the regression equation of benzoic acid was Y=586 238.6X+5 780,r=0.999 9,which was linear over the range of 0.249-2.490 μg, the average recovery was 99. 6%(n=9, RSD=0.38%); the regression equation of salicylic acid was Y=460 688.3X+694.6,r=1.000 0, which was linear over the range of 0.123-1.230 μg, the average recovery was 99.5%(n=9, RSD=0.70%). Conclusion:The method is sensi- tive, rapid, accurate, and can be used to control the quality of Wanxuanjing.