Myelinated Axons Demonstrated in the CNS and PNS by Anti-Neurofilament Immunoreactivity and Luxol Fast Blue Counterstaining

A method which demonstrates myelinated axons in the central and peripheral nervous systems will be described. Paraffin, frozen or semi-thin epoxy-embedded sections were immunostained first with a monoclonal antibody raised against a 200 kilo-Dalton neurofilament protein and then counterstained with Luxol fast blue