PC9 cell strain knocked down or over-expressed by TAZ as well as construction method and application of PC9 cell strain

The invention discloses an shRNA (Short Hairpin Ribonucleic Acid) molecule of a PC9 cell strain knocked down by a TAZ factor, a recombinant vector of the shRNA molecule, the recombinant vector of the PC9 cell strain over-expressed by the TAZ factor, and an establishment method of the PC9 cell strain knocked down and over-expressed by the TAZ. The method comprises the following steps: establishing the PC9 cell strain knocked down by the TAZ by transfecting PC9 cells via TAZ-shRNA by adopting an shRNA technology; establishing the PC9 cell strain over-expressed by the TAZ by transfecting the PC9 cells via a TAZ expression vector; detecting the TAZ in the cell strain by adopting an immune cell fluorescence technology, a RT-PCR (Reverse Transcription-Polymerase Chain Reaction) technology and a Western blot technology so as to prove the successful establishment of the cell strain; and measuring a cell growth curve and the cell doubling time. Thus, the powerful experimental material for researching the functions of the TAZ in the targeted therapy sensitivity of lung cancers is provided on the basis of the establishment of the PC9 cell strain knocked down and over-expressed by the TAZ.