Cloning and bioinformatics analysis of PsSFBB gene in Xinjiang pear.

[Objective] This study aimed to clone the PsSFBB gene from Xinjiang pear for bioinformatics analysis.[Method] PsSFBB gene was cloned from anthers of Qipan pear by using RT-PCR and RACE technologies for bioinformatics analysis.[Result] A SFBB gene with a full-length of 1 231 bp was cloned and named PsSFBB6-α(Genbank accession number: EU909685).PsSFBB6-α gene encoded a protein of 378 amino acids,with an F-box motif composed of about 50 amino acids in the N-terminal.According to the bioinformatics analysis,the molecular formula of PsSFBB6-α protein was C2000H3034N517O558S23,with relative molecular mass of 43 987.5 and isoelectric point of 6.02,and the secondary structure was dominated by α-helices;theoretically,the half life period was 30 h and the instability parameter was 55.21,so PsSFBB6-α protein was an instable protein;in addition,it was predicted that PsSFBB6-α protein was a hydrophilicity and non-secretory protein with lyases activity and specifically recognized substrates,which was consistent with the function of F-box protein.[Conclusion] This study had laid the foundation for further research of SFBB proteins and the mechanism of self-incompatibility and provided a theoretical basis for breeding of self-compatible cultivars of Xinjiang pear and scientific arrangement of pollination trees in production to increase the yield and quality.