Abstract 350: High-Mobility Group Protein B2 is Essential in Maintaining Normal Cardiac Gene Expression

Heart hypertrophy is a complex disease that involves differential expression of hundreds of genes and requires highly coordinated chromatin remodeling events that must facilitate such genome-wide changes in gene expression. We examine the genome-wide distribution of a chromatin structural protein, High-Mobility Group Protein B2 (HMGB2) in isolated cardiac myocytes with or without adrenergic receptor agonist. We report a comprehensive map of HMGB2 binding in both normal and hypertrophic cardiac myocytes, and find that HMGB2 preferentially localizes to promoters, CpG islands, enhancers and transcription factor binding sites. Moreover, we find that upon hypertrophic stimulation, HMGB2 peaks move from regulatory elements to intergenic regions. Because both HMGB2 knock-down and PHE treatment can induce hypertrophic growth, we compare gene expression dynamics between HMGB2 knock-down and PHE treatment, and find hypertrophy-related gene expression pattern upon HMGB2 knock-down is different from the one after PHE...