A role for PKCδ in foam cell formation: reply

We would like to thank Drs Katka Szilagyi and Timo K. van den Berg for their interest and comments regarding our recent report.1 We also greatly appreciate sharing with their interesting and unpublished results about PKCδ. Indeed, the approach with siRNA can result in changes in protein levels of certain untargeted genes that are unrelated to silencing of the targeted gene.2 However, in our study, we used different sequences of shRNAs or siRNAs against PKCδ which shared no cross-recognition with PKCβ in different cell models (THP-1 cells and human primary macrophages) and showed that knockdown of PKCδ reduced PKCβ protein expression. Interestingly, it has been shown that there is compensatory increases in PKCδ expression and activation in hearts of PKCɛ knock-out …