Development of Immunoenzyme Assay for Detection of Soybean Raw Material in Food Products

An enzyme-linked immunosorbent assay (ELISA) has been developed to control the presence and estimate the content of soybean raw materials in meat food products. It is based on the binding of specific antibodies to the antigenic determinants of a soybean trypsin inhibitor. Due to the possible influence of enzymatic and high-temperature product treatments that can disrupt the native structure of the inhibitor and lead to its fragmentation, the analysis is based on the competitive interaction with antibodies of the antigen in the sample, which are immobilized in microplate wells. The conditions providing the minimum detection limit and high measurement accuracy were established to implement the ELISA. The developed assay was characterized by a duration of 60 min, a detection limit of 3.5 ng/mL, and a range of detectable concentrations of 7.7–110 ng/mL. It is shown that the proposed methodology can be used for the testing meat products.