Ultrafast Fluorescence Signals from NADH: Resonant Energy Transfer in the Folded and Unfolded Forms

2020 
β-Dihydronicotinamide adenine dinucleotide (NADH) plays a critical role in biological redox processes. Inside the cell, NADH can be in a folded or unfolded conformation depending on the chemical environment that surrounds it. It is known that selective excitation of adenine in NADH can induce energy transfer events from this nucleotide to the reduced nicotinamide chromophore. From the anticipated time scales, this process competes with adenine’s ultrafast internal conversion channel, which is known to occur in the sub-picosecond time-scale for adenine. In this work we studied the dynamics of the excited states of both chromophores in NADH through the time resolution of the spontaneous emission from both chromophores. Through these experiments, we extend the knowledge about the competition between the different photophysical channels both in the folded and unfolded states. The study involved the folded and unfolded states of NADH through by experiments in water and methanol solutions. Our femtosecond fluor...
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