ANALYSIS OF GONAD PROTEOMES OF DIFFERENT GENDER SEA URCHIN STRONGYLOCENTROTUS NUDUS BY TWO-DIMENSIONAL ELECTROPHORESIS

We extracted total proteins in male and female gonads of sea urchin Strongylocentrotus nudus by the method of ultrasonication-acetone sedimentate-50mmol/L Tris-HCl(pH 6.8) and then separated by the carrier ampholytes two-dimensional electrophoresis in pH gradient. Results show that:(1) in pH 5-8, proteins in male and female gonads contained 321 and 402 protein spots, respectively;(2) in pH 4—6, they were 239 and 197 protein spots, respectively. Two hundred and eight unmatched protein spots were found in male and female gonad in pH 4—6 by PDQuest8.0 image analysis software, which suggests that there is a significant difference in protein expression level between male and female gonad of S. nudus.