Dietary fatty acids from pomegranate seeds (Punica granatum) inhibit adipogenesis and impact the expression of the obesity-associated mRNA transcripts in human adipose-derived mesenchymal stem cells
2019
have indicated that the pomegranate (Punica granatum) seed oil (PSO) has many biologically
active components that help in controlling diet‐induced obesity and insulin
resistance. However, its impact on the adipogenic differentiation of human adiposederived
mesenchymal stem cells (HADMSC) remains unclear. Here we have attempted
to study the anti‐obesity potential of SHAMstat3pg, a fatty acid composite
extracted from PSO. It is composed of three dietary fatty acids: punicic acid
[(9Z,11E,13Z)‐9,11,13‐Octadecatrienoic acid], oleic acid [Cis‐9‐Octadecenoic acid],
and linoleic acid [(9Z,12Z)‐octadeca‐9,12‐dienoic acid]. In this study, we discuss the
impact of the fatty acids on adipogenesis, inflammation, glucose uptake, and mitochondrial
ATP production. The impact of SHAMstat3pg on the expression of various
obesity‐associated protein and mRNA transcripts in HADMSC was also analyzed.
The results indicate that exposure to 10 μg/ml of SHAMstat3pg (24 hr) inhibited adipogenesis
of HADMSC, ameliorated inflammation, attenuated ATP production, and
glucose uptake. Also, the extract favorably regulated the mRNA expression of the
studied obesity‐associated gene transcripts.
Practical applications
SHAMstat3pg has the potential to serve as a multi‐targeted therapy for the management
of obesity. This study demonstrated that the dietary fatty acids inhibited the
differentiation of preadipocytes to adipocytes. SHAMstat3pg has also shown to have
a favorable impact on the expression of the obesity‐linked proteins and genes in
HADMSC that are associated with adipogenesis, inflammation, satiety, energy intake/
expenditure (central and peripheral signaling molecules). The study gives an
overview of the vast number of genes impacted by the treatment with SHAMstat3pg
paving the way for future studies to demonstrate the exact mode of action of how
dietary fatty acids can help manage obesity, insulin resistance, and type 2 diabetes.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
56
References
4
Citations
NaN
KQI