Expression of DLC1 gene in human glioma tissues

Objective To investigate the expression and the role of DCL1 gene in human glioma. Methods Thirty-nine glioma samples and 10 control tissues resected from healthy brain tissue with traumatic brain injury were collected for analysis. Real-time quantitative PCR was employed to detect the DCL1 gene expression at the mRNA level. Plasmid pCS2-DLC1was transfected into the glioma cell line U251 and pCS2-MT was transfected as control group; Western blotting was employed to detect the DCL1 gene expression at the protein level: the expression of Myc; MTT assays were performed to assess the proliferation of glioma cell line U251. Results Glioma tissues showed a significant increased DCL1 gene expression at mRNA level as compared with the control brain tissues (P=0.000). The DLC1 gene expression at mRNA level in the Grade Ⅰ gliomas was obviously higher than that in Grade Ⅱ,Ⅲ or Ⅳ ones (P=0.001). Over-expression of Myc in pCS2-DLC1 transfected group was found, while no obvious Myc expression was noted in the control group. The pCS2-DLC1 transfected group showed higher proliferation level of U251 cell lines than the pCS2-MT control group (P=0.002). Conclusion High expression level of DLC1 might contribute to the formation and development of glioma. Key words: Glioma; DLC1 gene; Proliferation