Study on the role of flavonoids derived extract from seed residues of hippophae rhamnoides on high-fat diet induced obese mice

Abstract The objective of the paper was to explore the active mechanism underlying the role of flavonoids, which were derived from seed residues of hippophae rhamnoides (Flavonoids from the Seeds of H. rhamnoides L., FSH), in obesity. A total of 50 SPF healthy male C57BL/6 mice were selected and divided into 5 groups (with 10 mice in each group), i.e., the normal control group, the high fat diet control group, the 100mg/kg FSH group, the 300mg/kg FSH group, and the rosiglitazone group. The basic biochemical indicators of the mice were detected, and the liver and white adipose tissue samples were collected, stained and analyzed via Western Blot to study the effects of flavonoids on obesity. In the experiment, rosiglitazone was used as a traditional weight-inhibiting drug and its efficacy was compared with that in the FSH groups. It was found that compared with that in the high-fat diet control group, the weight gain of obese mice administered with FSH was slowed, along with reduced levels of serum total cholesterol, plasma glucose, and liver fat accumulation. Meanwhile, it was also discovered that FSH could reduce the expression of PPARγ in liver and white adipose tissues, thus helping to reduce fat accumulation. It was proved that the flavonoids derived from seed residues of hippophae rhamnoides exerted an inhibitory effect on obesity to protect the liver.