INOSINE FERMENTATION-PRODUCTION OF INOSINE BY MUTANTS OF BACILLUS SUBTILIS

Using rab values, rates of oxygen transfer into sulfite solution, as an index for establishing aeration-agitation, the fermentative production of inosine was scaled up from a flask culture to a 50-k1 tank culture. By applying the optimum condition for the production of inosine obtained with small scale fermentors, the yield of inosine obtained with a 50-k1 fermentor was approximately the same as that from small-scale fermentors. With due consideration to cotton plugs as a barrier of oxygen transfer, the advantage of using rab values for aeration-agitation index and for scale-up of aerobic fermentation was discussed.