2, 4-dihydroxy-5-bromo-alpha-phenylacetophenone Thiosemicarbazone (DHB-and-PAT) as Analytical Reagent: Studies on Cu(II) Chelate

dihydroxy -5-bromo-α-phenyl acetophenone thiosemicarbazone (DHB-α-PAT) has been used for the spectrophotometric determination of Cu(II). The method is simple, rapid and sensitive. Light green colour 1:2 (M:L) complex formed between Cu(II) and DHB-α-PAT in the pH range 3.0-6.5. The absorption spectrum shows as shoulder at 420 nm. The molar absorptivity and Sandell's sensitivity is found to be 5.018 x10 3 lit.mol -1 .cm -1 and 0.01266 µg/cm 2 , respectively. A number of foreign ions are tested for their interference. Antibacterial activity, TGA studies, Elemental analysis, 1H 1 NMR spectra of Reagent, IR spectra of the reagent and its complex were reported. The reagent has been successfully applied to the determination of Cu(II) in drained microetch solution.