Preparation and inhibition on α-d-glucosidase of low molecular weight polysaccharide from Cordyceps militaris
2016
Abstract The structural properties and the inhibition on α- d -glucosidase activity of the low molecular weight (LCMPs - II) obtained from the optimized acid hydrolysis of the Cordyceps militaris polysaccharides (CMPs) were investigated in this paper. The LCMPs - II with a molecular weight of 28 KDa mainly composed of rhamnose, xylose and glucose with the molar ratio of 1: 2.19: 6.73 was separated from LCMPs-I which was the acid hydrolysis product of CMPs by chromatography on Sephadex G-100 column. The solubility of LCMPs - II was tested to be 32.12 ± 1.05 g in 100 mL distilled water under 25 °C. Its solubility was almost as twice as that of CMPs. Afterward, the structural features of LCMPs - II was investigated by a combination of chemical and instrumental analysis such as the specific rotation determination, FT-IR, periodate oxidation-Smith degradation, Congo-red, GC, scanning electron microscope and NMR. The results showed that the optical rotation of LCMPs - II was +25° and it was 1,3-branched-rhamnoxyloglucan which had a linear backbone of (1 → 4)-linked α- d -glucopyranose (α- d -Glc p units).
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