Development and characterization of SNP markers in many-lined sun skink (Eutropis multifasciata) from transcriptomic sequences

Many-lined sun skink (Eutropis multifasciata) is a medium sized viviparous scincid lizard. Insufficient molecular markers have limited the effective conservation and management of E. multifasciata. In the present study, 28 novel single nucleotide polymorphism (SNP) markers were developed from the transcriptome dataset of E. multifasciata using SNaPshot method. The minor allele frequency ranged from 0.117 to 0.483. The observed heterozygosity and expected heterozygosity ranged from 0.167 to 0.567 and from 0.210 to 0.508, respectively. Polymorphic information content ranged from 0.185 to 0.375. None of the loci deviated significantly from Hardy–Weinberg equilibrium and no significant linkage disequilibrium was found. Those polymorphic SNP markers will be helpful for the further studies on population structure and conservation genetics in E. multifasciata.