Protective effect of Kinsenoside on acute alcohol-induced liver injury in mice

Abstract Anoectochilus roxburghii is a Chinese medicinal plant which can be effective for some diseases such as hepatitis, nephritis, pneumonia. Its active ingredient is Kinsenoside (KD). The mechanisms of KD on the liver-protective effect have not been fully explored until today. The present study was aimed to investigate the protective effect and mechanism of Kinsenoside on acute alcoholic liver injury. The protected activity of KD (10, 20 and 40 mg/kg) were investigated on acute alcoholic liver injury in mice. Male C57BL/6 J mice were fed with non-fat feed for 30 days and oral administrated 14 mL/kg BW of ethanol (50%) on the 31st day. The activities of AST, ALT, TG and VLDL were determined in serum. The hepatic levels of GSH, MDA were measured in liver homogenates. The levels of cytochrome P450 2E1 (CYP2E1) were measured by immunohistochemistry. Furthermore, histopathological observations were carried out on the separated livers of mice. It was suggested that the trends of acute hepatic injury and fatty degeneration induced by alcohol were reduced in the ethanol group after KD treatment. Compared to ethanol groups, TG, MDA, VLDL, GSH, ALT and AST levels of KD (20, 40 mg/kg) groups were decreased ( P