EinfluB des Pneumoperitoneums auf die Expression von Adhasionsmolekiilen auf HT-29 Kolonkarzinomzellen Impact of pneumoperitoneum on expression of cell-adhesion molecules on HT-29 colon-carcinoma cells

Themechanismofpotentialtumorcellspreadduringlaparoscopyis poorlyunderstood. Itis speculatedthattheincreasedintraabdominalpressureandtheuseofcarbondioxideduringlapa­ roscopychangethemetastaticpotentialofcarcinomacells.Thestudyinvestigatedtheinfluenceof CO 2 andheliuminsufflationanddifferentpressure(10mmHg/20mmHg)ontheinvitroexpres­ sionofE-Cadherin,CD44v6,ICAM-Iand/3I-IntegrinonHT-29coloncarcinomacells.Theadhe­ sionmoleculeexpressionwasmeasuredI,12,24,48and96hafterCO2andheliuminsufflation using flowcytometry (FACScan). HT-29 cell line showed a short significant decrease in E­ Cadherin,CD44v6,ICAM-Iand/31-integrinexpressionafterCO 2exposure.Heliuminsufflation hadonlyinfluenceontheexpressionof/3l-integrin.Whetherthesechangesincreasethemetastatic potentialoftumorcellsinvivoneedsfurtherinvestigation.