SYNTHESIS, HYBRIDIZATION, AND NUCLEASE RESISTANCE PROPERTIES OF 2'-O-AMINOOXYETHYL MODIFIED OLIGONUCLEOTIDES

Abstract We have synthesized the novel 2′-O-AOE- and MIOE-5-methyluridine and -adenosine nucleosides and successfully incorporated them into oligonucleotides. The 2′-O-modifications significantly enhance hybridization against RNA (1.2 deg C/substitution) and furthermore, exhibits specificity for RNA vs. DNA. The nuclease resistance (SVPD) of 2′-O-AOE and MIOE modified oligonucleotides is comparable to that of 2′-O-MOE.